Adaptation of Very Virulent Infectious Bursal Disease Virus to Chicken Embryonic Fibroblasts by Site-Directed Mutagenesis of Residues 279 and 284 of Viral Coat Protein VP2

Vaccination against very virulent infectious bursal disease virus using recombinant T4 bacteriophage displaying viral protein VP2.

In order to develop a desirable inexpensive, effective and safe vaccine against the very virulent infectious bursal disease virus (vvIBDV), we tried to take advantage of the emerging T4 bacteriophage surface protein display system. The major immunogen protein VP2 from the vvIBDV strain HK46 was fused to the nonessential T4 phage surface capsid protein, a small outer capsid (SOC) protein, result...

Expression, purification and glycosylation analysis of chicken infectious bursal disease virus VP2 in yeast

Infectious bursal disease (IBD), a highly contagious and devastating disease in young chicken, is causedby infectious bursal disease virus (IBDV). To improve the immunogenicity of recombinant IBDV subunitvaccine, an attempt was made to find a new way to prepare IBD vaccine containing glycosylated mVP2antigen. Firstly, IBDV mVP2 gene (with a nucleic acid sequence encoding B cell epitope of IBDV(...

Very Virulent Infectious Bursal Disease Virus

The primary feature of vvIBDV is the ability to induce high mortality. Mortality ranges from 5% – 25% in broilers and 30% – 70% in layers. Surviving birds are severely affected with a high condemnation rate. vvIBDV induces clinical signs similar to Gumboro disease, which is induced by classical virulent strains of IBDV, except that the disease is more pronounced and acute in individual birds an...

Characterization of a Highly Virulent Infectious Bursal Disease Virus

Sequence analysis of the VP1 gene in three very virulent Iranian infectious bursal disease virus strains

Infectious bursal disease (IBD) is a highly contagious disease of chickens caused by the infectious bursal disease virus (IBDV). This study was conducted to characterize three IBDV strains from Iran. A reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was used to amplify a 715-bp fragment of the VP1 gene from IBDV strains. Amplified VP1 fragments of the three Iranian IBDV strai...